Friday, May 8, 2015

A Brief History of Genetics

Recent events have demonstrated the public's pronounced lack of knowledge regarding genetics and genetic engineering. While it is unnecessary to possess a doctorate in order to understand the intricacies of this rapidly-evolving science, it is important to have a framework to consider the various challenges to societal norms and biological changes to the population at large that genetics have created.
To truly begin understanding a subject, context is always necessary. To serve that end, here is a brief timeline outlining the brief but paradigm shifting history of genetics:
1858: Charles Darwin and Alfred Russel Wallace jointly announce the theory of natural selection, which postulates that members of a population, whether plant or animal, who better adapt to their natural environment not only survive but pass on their traits to successive generations.
1859: Charles Darwin publishes "On the Origin of Species by Means of Natural Selection, or the Preservation of Favored Races in the Struggle for Life". The work causes a stir in religious circles and society at large, as it advances Darwin's theory of evolution to the masses.
1865: Gregor Mendel's experimentation with peas leads to his discovery of heredity and its transmission through discrete units. His research demonstrated the idea that genes remain as distinct entities, even if they create a blended-trait progeny. This ability to combine specific, distinct traits into new organisms provides further support for Darwin's theory of natural selection and evolution.
1869: Friedrich Miescher isolates DNA itself from cells for the first time and calls the mysterious substance "nuclein", while investigating pus in open wounds. This substance would later be referred to as "nucleic acid" after Miescher was able to separate the genetic material into a protein and acid molecule.
1879: Walter Flemming discovers the cellular process of mitosis, while observing chromosomes during animal cell divisions. He would later collect his observations and detail the entire process in 1882.
1900: Botanists Hugo de Vries, Carl Correns, and Erich von Tschermak each rediscover Mendel's work while conducting their own research regarding biological inheritance. The increased understanding of cells and chromosomes, due to work from people like Walter Flemming, allowed Mendel's concepts to finally have concrete examples as illustrations.
1902: Walter Sutton observes that the segregation of chromosomes during cellular meiosis matches the inheritance patterns Mendel observed, thus demonstrating that chromosomes play an integral role in passing on physical traits to organisms.
1905: Nettie Stevens and Edmund Wilson independently observe and describe the behavior of sex chromosomes, verifying that XX determines a female animal while XY determines a male animal.
1909: Wilhelm Johannsen creates the word "gene" to finally quantify Mendel's distinctive trait units. He also devises the terms "genotype" and "phenotype" in order to distinguish the differences between an individual's inner, genetic traits and outward appearance characteristics.
1911: Thomas Hunt Morgan study fruit fly chromosomes and discover that chromosomes, in fact, are the structures that carry genes. This work also leads to the formulation of genetic linkage theory.
1927: Hermann J. Muller uses X-rays to induce artificial gene mutations in fruit flies; after observing the results of this accident, he formulates the principles of spontaneous gene mutation in plants and animals.
1941: George Beadle and Edward Tatum's experiments with irradiating red bread mold, leads to the discovery that genes function by regulating specific chemical reactions. They hypothesize that each specific gene is responsible for the generation of an associated enzyme, which affects the organism's body accordingly.
1944: Oswald Avery, Colin MacLeod, and Maclyn McCarty demonstrate that DNA itself can adjust and change a cell's properties, which in turn clarified the chemical governing abilities of genes that Beadle and Tatum uncovered.
1951: Rosalind Franklin obtains the first clear X-ray diffraction photographs of DNA itself; her work would prove instrumental in solving DNA's structure despite being overlooked publicly for decades.
1953: Building upon the photographic work of Rosalind Franklin, Francis Crick and James Watson uncover and describe the now-famous 'double helix' structure of DNA. Their efforts would lead to receiving the Nobel Prize in 1962.
1955: Joe Hin Tijo is able to determine that every human cell contains exactly 46 chromosomes, broken apart into 23 distinctive pairs.
1966: Hamilton Smith and Kent Wilcox discover and isolate the first known restriction enzyme, HindII. These enzymes are able to cut DNA molecules down into segments via specific sites. This knowledge would eventually lead to DNA sequence analysis and genome mapping.
1972: Herb Boyer and Paul Berg create the first recombinant DNA molecules, in which DNA from separate species are combined together. The resultant hybrid molecule is then inserted into a host cell. This would eventually lead to the production of the first recombinant DNA medication, human insulin.
1975: Frederick Sanger develops the Sanger method, which utilizes colored dyes to identify the four specific nucleic acids that compose DNA. As a result, DNA sequencing is born, allowing scientists to begin uncovering the specific structures and functions of various genes.
1976: Genentech, the first genetic engineering company, is founded by Herb Boyer utilizing his recombinant DNA technology to produce human insulin thereby launching biotech.
1988: The Human Genome Project is formed, with its mission of determining the entire sequence of DNA that all human chromosomes are composed of in every cell.
1989: Alec Jeffreys coins the term "DNA fingerprinting" and was the first researcher to apply DNA analysis technology towards paternity, immigration, and murder cases.
1993: FlavrSavr tomatoes, the first genetically-modified organisms ("GMO") available for public purchase and consumption, come onto the market. They are engineered to have a longer shelf life than naturally grown tomatoes.
1997: GeneTree, the first company to use DNA testing to trace ancestry is launched. In time, various other companies would enter the ancestry testing space.
2000: The Human Genome Project unveils its initial results; 90% of the human genome is fully sequenced and deemed to be 99.9% accurate after additional testing and checking.
There are a myriad of other events that have helped shape this scientific field, however by examining this particular chain of history certain trends emerge. First, the investigation into life's building blocks began well before the hugely publicized discover of the double helix in 1953. Second, as with most other movements, every major breakthrough stands upon the shoulders of previous pioneers who may not have been able to see the entire picture clearly but understood that the pieces they found were important. This faith in progress and constant revisiting of the past has led to breakthroughs in genetics that continue changing society on a daily basis.
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